Facile fabrication of hydrophilic covalent organic framework composites for highly selective enrichment of N-glycopeptides

Yichen Lu; Chengrun Du; Hongmei Ying; Yunfan Lin; Qinying Gu; Fangfang Kong; Hongli Zhao; Minbo Lan

doi:10.1016/j.talanta.2023.124524

Facile fabrication of hydrophilic covalent organic framework composites for highly selective enrichment of N-glycopeptides

Talanta. 2023 Jul 1:259:124524. doi: 10.1016/j.talanta.2023.124524. Epub 2023 Apr 6.

Authors

Yichen Lu¹, Chengrun Du², Hongmei Ying³, Yunfan Lin¹, Qinying Gu¹, Fangfang Kong², Hongli Zhao¹, Minbo Lan⁴

Affiliations

¹ Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, China.
² Department of Radiation Oncology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China; Shanghai Clinical Research Center for Radiation Oncology, Shanghai, 200032, China; Shanghai Key Laboratory of Radiation Oncology, Shanghai, 200032, China.
³ Department of Radiation Oncology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China; Shanghai Clinical Research Center for Radiation Oncology, Shanghai, 200032, China; Shanghai Key Laboratory of Radiation Oncology, Shanghai, 200032, China. Electronic address: yinghongmei2013@163.com.
⁴ Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, China. Electronic address: minbolan@ecust.edu.cn.

PMID: 37054624
DOI: 10.1016/j.talanta.2023.124524

Abstract

The development of facilely synthetic materials acts an essential role in glycoproteome analysis, especially for the highly efficient enrichment of N-linked glycopeptides. In this work, a facile and timesaving route was introduced in which COF_TP-TAPT served as a carrier and poly (ethylenimine) (PEI) and carrageenan (Carr) were successively coated on the surface via electrostatic interaction. The resultant COF_TP-TAPT@PEI@Carr showed remarkable performance in glycopeptide enrichment with high sensitivity (2 fmol μL^-1), high selectivity (1:800, molar ratio of human serum IgG to BSA digests), large loading capacity (300 mg g^-1), satisfactory recovery (102.4 ± 6.0%) and reusability (at least eight times). Due to the brilliant hydrophilicity and electrostatic interactions between COF_TP-TAPT@PEI@Carr and positively charged glycopeptides, the prepared materials could be applied in the identification and analysis in the human plasma of healthy subjects and patients with nasopharyngeal carcinoma. As a result, 113 N-glycopeptides with 141 glycosylation sites corresponding to 59 proteins and 144 N-glycopeptides with 177 glycosylation sites corresponding to 67 proteins were enriched from 2 μL plasma trypsin digests of the control groups and patients with nasopharyngeal carcinoma, respectively. 22 glycopeptides were identified only from the normal controls and 53 glycopeptides were detected only from the other set. The results demonstrated that this hydrophilic material was promising on a large scale and further N-glycoproteome research.

Keywords: Covalent organic frameworks; Glycopeptides enrichment; Hydrophilicity; Stability.

MeSH terms

Glycopeptides / analysis
Humans
Hydrophobic and Hydrophilic Interactions
Immunoglobulin G
Metal-Organic Frameworks*
Nasopharyngeal Carcinoma
Nasopharyngeal Neoplasms*

Substances

Glycopeptides
Metal-Organic Frameworks
Immunoglobulin G