CRISPR screening in cardiovascular research

Haihuan Shan; Teng Fei

doi:10.3389/fcell.2023.1175849

CRISPR screening in cardiovascular research

Front Cell Dev Biol. 2023 Apr 12:11:1175849. doi: 10.3389/fcell.2023.1175849. eCollection 2023.

Authors

Haihuan Shan^{1

2}, Teng Fei^{1

2}

Affiliations

¹ National Frontiers Science Center for Industrial Intelligence and Systems Optimization, Key Laboratory of Bioresource Research and Development of Liaoning Province, College of Life and Health Sciences, Northeastern University, Shenyang, China.
² Key Laboratory of Data Analytics and Optimization for Smart Industry (Northeastern University), Ministry of Education, Shenyang, China.

Abstract

The recent advent and widespread application of CRISPR-based genome editing tools have revolutionized biomedical research and beyond. Taking advantage of high perturbation efficiency and scalability, CRISPR screening has been regarded as one of the most powerful technologies in functional genomics which allows investigation of different genetic subjects at a large scale in parallel. Significant progress has been made using various CRISPR screening tools especially in cancer research, however, fewer attempts and less success are reported in other contexts. In this mini-review, we discuss how CRISPR screening has been implemented in studies on cardiovascular research and related metabolic disorders, highlight the scientific progress utilizing CRISPR screening, and further envision how to fully unleash the power of this technique to expedite scientific discoveries in these fields.

Keywords: CRISPR; cardiovascular; disease; screen; screening.

Publication types

Review

Grants and funding

This work was supported by the National Natural Science Foundation of China (31871344; 32071441), the Fundamental Research Funds for the Central Universities (N182005005), the 111 Project (B16009), and Key Laboratory of Bioresource Research and Development of Liaoning Province (2022JH13/10200026).