Endoplasmic reticulum stress impairs the immune regulation property of macrophages in asthmatic patients

Lihua Mo; Yu Liu; Xuejie Xu; Xinxin Wang; Shuang Zhang; Suqin Hu; Yongjin Wu; Zhiyuan Tang; Qinmiao Huang; Jing Li; Xizhuo Sun; Pingchang Yang

doi:10.1016/j.clim.2023.109639

Endoplasmic reticulum stress impairs the immune regulation property of macrophages in asthmatic patients

Clin Immunol. 2023 Jul:252:109639. doi: 10.1016/j.clim.2023.109639. Epub 2023 May 10.

Authors

Lihua Mo¹, Yu Liu², Xuejie Xu³, Xinxin Wang³, Shuang Zhang³, Suqin Hu², Yongjin Wu⁴, Zhiyuan Tang⁴, Qinmiao Huang², Jing Li⁵, Xizhuo Sun⁶, Pingchang Yang⁷

Affiliations

¹ Department of General Practice Medicine and Respirology, Third Affiliated Hospital of Shenzhen University, Shenzhen, China; Guangdong Provincial Regional Disease Key Laboratory, Shenzhen, China; Institute of Allergy & Immunology of Shenzhen University, State Key Laboratory of Respiratory Diseases Allergy Division at Shenzhen University, Shenzhen, China.
² Department of General Practice Medicine and Respirology, Third Affiliated Hospital of Shenzhen University, Shenzhen, China.
³ Guangdong Provincial Regional Disease Key Laboratory, Shenzhen, China; Institute of Allergy & Immunology of Shenzhen University, State Key Laboratory of Respiratory Diseases Allergy Division at Shenzhen University, Shenzhen, China.
⁴ Department of Allergy, Longgang ENT Hospital, Shenzhen, China.
⁵ Department of Allergy, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. Electronic address: lijing@gird.cn.
⁶ Department of General Practice Medicine and Respirology, Third Affiliated Hospital of Shenzhen University, Shenzhen, China. Electronic address: xizhoursun@126.com.
⁷ Guangdong Provincial Regional Disease Key Laboratory, Shenzhen, China; Institute of Allergy & Immunology of Shenzhen University, State Key Laboratory of Respiratory Diseases Allergy Division at Shenzhen University, Shenzhen, China. Electronic address: pcy2356@szu.edu.cn.

PMID: 37172666
DOI: 10.1016/j.clim.2023.109639

Abstract

The current study aims to characterize the counteraction of M2 cells in response to Endoplasmic reticulum (ER) stress. ER stress was detected in bronchoalveolar lavage fluids (BALF) Mϕs, which was at unresolved state in asthma patients. A positive correlation was detected between ER stress in Mϕs and lung functions/allergic mediators/Th2 cytokines in BALF or specific IgE in the serum. Levels of immune regulatory mediator in the BALF were negatively correlated to ER stress in BALF Mϕs. The ER stress state influenced the immune regulatory property of BALF Mϕ. Exposure to environmental pollutant, 3-metheyl-4-nitrophenol, exacerbated ER stress in Mϕ, which affected the Mϕ phenotyping. Exacerbation of ER stress suppressed the expression of IL-10 and programmed cell death protein-1 (PD-1) in Mϕs by increasing the expression of the ring finger protein 20 (Rnf20). Conditional inhibition of Rnf20 in Mϕs attenuated experimental airway allergy.

Keywords: Air pollution; Allergy; Endoplasmic reticulum stress; Immune regulation; Macrophage.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Asthma*
Bronchoalveolar Lavage Fluid
Cytokines
Disease Models, Animal
Endoplasmic Reticulum Stress
Humans
Lung
Macrophages
Mice
Mice, Inbred BALB C

Substances

Cytokines