E15.5 Mouse Embryo Micro-CT Using a Bruker Skyscan 1172 Micro-CT

Elena Astanina; Sara Petrillo; Tullio Genova; Federico Mussano; Federico Bussolino

doi:10.21769/BioProtoc.4662

E15.5 Mouse Embryo Micro-CT Using a Bruker Skyscan 1172 Micro-CT

Bio Protoc. 2023 May 5;13(9):e4662. doi: 10.21769/BioProtoc.4662.

Authors

Elena Astanina^{1

2}, Sara Petrillo³, Tullio Genova⁴, Federico Mussano⁵, Federico Bussolino^{1

2}

Affiliations

¹ Department of Oncology, University of Torino, Torino, Italy.
² Candiolo Cancer Institute-IRCCS-FPO, Candiolo, Italy.
³ Department of Molecular Biotechnology and Health Sciences, Molecular Biotechnology Center, University of Torino, Torino, Italy.
⁴ Department of Life Sciences and Systems Biology, University of Torino, Torino, Italy.
⁵ CIR Dental School, Department of Surgical Sciences, University of Torino, Via Nizza 230, 10126, Torino, Italy.

Abstract

X-ray computed microtomography (µCT) is a powerful tool to reveal the 3D structure of tissues and organs. Compared with the traditional sectioning, staining, and microscopy image acquisition, it allows a better understanding of the morphology and a precise morphometric analysis. Here, we describe a method for 3D visualization and morphometric analysis by µCT scanning of the embryonic heart of iodine-stained E15.5 mouse embryos.

Keywords: 3D visualization; Heart development; Imaging; Micro-CT; Mouse embryo; X-ray computed tomography.