Mussel-inspired HA@TA-CS/SA biomimetic 3D printed scaffolds with antibacterial activity for bone repair

Cheng Ji; Chengcheng Zhang; Zeya Xu; Yan Chen; Yanming Gan; Minghui Zhou; Lan Li; Qinying Duan; Tingting Huang; Jinxin Lin

doi:10.3389/fbioe.2023.1193605

Mussel-inspired HA@TA-CS/SA biomimetic 3D printed scaffolds with antibacterial activity for bone repair

Front Bioeng Biotechnol. 2023 May 9:11:1193605. doi: 10.3389/fbioe.2023.1193605. eCollection 2023.

Authors

Cheng Ji^{1

2

3

4}, Chengcheng Zhang⁵, Zeya Xu^{1

2}, Yan Chen^{1

2}, Yanming Gan^{1

2

4}, Minghui Zhou^{1

2

4}, Lan Li^{1

2}, Qinying Duan^{1

2

4}, Tingting Huang^{1

2}, Jinxin Lin^{1

2

3}

Affiliations

¹ Quanzhou Institute of Equipment Manufacturing, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Quanzhou, Fujian, China.
² Fujian Science and Technology Innovation Laboratory for Optoelectronic Information of China, Fuzhou, Fujian, China.
³ College of Chemistry and Materials Science, Fujian Normal University, Fuzhou, China.
⁴ Fujian College, University of Chinese Academy of Sciences, Fuzhou, China.
⁵ Fujian Medical University, Fuzhou, China.

Abstract

Bacterial infection is a major challenge that could threaten the patient's life in repairing bone defects with implant materials. Developing functional scaffolds with an intelligent antibacterial function that can be used for bone repair is very important. We constructed a drug delivery (HA@TA-CS/SA) scaffold with curcumin-loaded dendritic mesoporous organic silica nanoparticles (DMON@Cur) via 3D printing for antibacterial bone repair. Inspired by the adhesion mechanism of mussels, the HA@TA-CS/SA scaffold of hydroxyapatite (HA) and chitosan (CS) is bridged by tannic acid (TA), which in turn binds sodium alginate (SA) using electrostatic interactions. The results showed that the HA@TA-CS/SA composite scaffold had better mechanical properties compared with recent literature data, reaching 68.09 MPa. It displayed excellent degradation and mineralization capabilities with strong biocompatibility in vitro. Furthermore, the antibacterial test results indicated that the curcumin-loaded scaffold inhibited S.aureus and E.coli with 99.99% and 96.56% effectiveness, respectively. These findings show that 3D printed curcumin-loaded HA@TA-CS/SA scaffold has considerable promise for bone tissue engineering.

Keywords: 3D printing; DMON; antibacterial; curcumin; scaffolds.

Grants and funding

This work was financially supported by the National Natural Science Foundation of China (No. 51801198 and No. 82072074), Funds of Scientific and Technological Plan of Fujian Province (Nos 2020Y0083 and 2020L3026) and Fujian Science and Technology Innovation Laboratory for Optoelectronic Information of China (No. 2021ZZ111).