Reconstitution of Human Prospermatogonial Development from Human-Induced Pluripotent Stem Cells

Yasunari Seita; Young Sun Hwang; Kotaro Sasaki

doi:10.1007/978-1-0716-3139-3_8

Reconstitution of Human Prospermatogonial Development from Human-Induced Pluripotent Stem Cells

Methods Mol Biol. 2023:2656:145-159. doi: 10.1007/978-1-0716-3139-3_8.

Authors

Yasunari Seita^{1

2}, Young Sun Hwang¹, Kotaro Sasaki³

Affiliations

¹ Department of Biomedical Sciences, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA, USA.
² Bell Research Center for Reproductive Health and Cancer, Nagoya, Aichi, Japan.
³ Department of Biomedical Sciences, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA, USA. ksasaki@upenn.edu.

PMID: 37249870
DOI: 10.1007/978-1-0716-3139-3_8

Abstract

There is a scarcity of information regarding the molecular mechanisms underlying human germ cell development due to limitations in obtaining the relevant materials. Reconstitution of human germ cell development from pluripotent stem cells in vitro would provide critical insight into the etiology of various reproductive conditions and disorders, including infertility.Recently, we reported the in vitro reconstitution of human prospermatogonial development from human-induced pluripotent stem cells through human primordial germ cell (PGC)-like cells (hPGCLCs) using long-term cultured xenogeneic reconstituted testes. Here, we describe a method to generate M-prospermatogonia-like cells (MLCs) and T1-prospermatogonia-like cells (T1LCs), which closely resemble M- and T1-prospermatogonia present in second-trimester human fetal testes in vivo.

Keywords: Human primordial germ cell-like cells; In vitro gametogenesis; Prospermatogonia; T1-prospermatogonia-like cells.

MeSH terms

Cell Differentiation
Germ Cells
Humans
Induced Pluripotent Stem Cells*
Male
Pluripotent Stem Cells*
Testis