Phagosomal pattern recognition receptor signaling promotes phagosome maturation and additional immune pathways such as proinflammatory cytokine secretion and antigen MHC-II presentation in antigen-presenting cells. In the present chapter, we describe procedures to assess these pathways in murine dendritic cells, professional phagocytes positioned at the interface between innate and adaptive immune responses. The assays described herein follow proinflammatory signaling by biochemical and immunological assays as well as antigen presentation of the model antigen Eα by immunofluorescence followed by flow cytometry.
Keywords: Antigen presentation; Dendritic cells; Eα; MHC-II; Phagocytosis.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.