Strategies and Progress of Raman Technologies for Cellular Uptake Analysis of the Drug Delivery Systems

Yajuan Liu; Mei Li; Haisha Liu; Chao Kang; Xiyong Yu

doi:10.2147/IJN.S435087

Strategies and Progress of Raman Technologies for Cellular Uptake Analysis of the Drug Delivery Systems

Int J Nanomedicine. 2023 Nov 20:18:6883-6900. doi: 10.2147/IJN.S435087. eCollection 2023.

Authors

Yajuan Liu¹, Mei Li², Haisha Liu², Chao Kang², Xiyong Yu¹

Affiliations

¹ Key Laboratory of Molecular Target & Clinical Pharmacology, and the NMPA & State Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences & the Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, 511436, People's Republic of China.
² School of Chemistry and Chemical Engineering, Guizhou University, Guiyang, 550025, People's Republic of China.

Abstract

Nanoparticle (NP)-based drug delivery systems have the potential to significantly enhance the pharmacological and therapeutic properties of drugs. These systems enhance the bioavailability and biocompatibility of pharmaceutical agents via enabling targeted delivery to specific tissues or organs. However, the efficacy and safety of these systems are largely dependent on the cellular uptake and intracellular transport of NPs. Thus, it is crucial to monitor the intracellular behavior of NPs within a single cell. Yet, it is challenging due to the complexity and size of the cell. Recently, the development of the Raman instrumentation offers a versatile tool to allow noninvasive cellular measurements. The primary objective of this review is to highlight the most recent advancements in Raman techniques (spontaneous Raman scattering, bioorthogonal Raman scattering, coherence Raman scattering, and surface-enhanced Raman scattering) when it comes to assessing the internalization of NP-based drug delivery systems and their subsequent movement within cells.

Keywords: coherence Raman scattering; drug delivery systems; nanoparticle; spontaneous Raman scattering; surface-enhanced Raman scattering.

Publication types

Review

MeSH terms

Drug Delivery Systems* / methods
Nanoparticles*
Pharmaceutical Preparations
Spectrum Analysis, Raman / methods

Substances

Pharmaceutical Preparations

Grants and funding

This research was supported by National Key Research and Development Program of China (2022YFE0209700), the grant from Bureau of Education of Guangzhou Municipality Foundation (No. 202201010404 and No.201831836), the Guizhou Provincial Science and Technology Project (No. ZK2022063), and plan on enhancing scientific research in GMU.