Persistence of dengue genome in a remotely infected patient

Soraya Thaivanich; Jirayu Visuthranukul; Kesinee Arunyingmongkol; Udomsak Bunworasate; Padet Siriyasatien; Wanla Kulwichit

doi:10.2478/abm-2023-0072

Persistence of dengue genome in a remotely infected patient

Asian Biomed (Res Rev News). 2023 Dec 28;17(6):287-290. doi: 10.2478/abm-2023-0072. eCollection 2023 Dec.

Authors

Soraya Thaivanich^{1

2}, Jirayu Visuthranukul^{1

2}, Kesinee Arunyingmongkol^{1

2}, Udomsak Bunworasate², Padet Siriyasatien^{1

3}, Wanla Kulwichit^{1

2}

Affiliations

¹ Clinical Excellence Center for Emerging Infectious Diseases, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok 10330, Thailand.
² Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
³ Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

Abstract

Background: Dengue virus infection is an intriguing illness. It is traditionally thought of as a self-limited and nonpersistent disease.

Objectives: We report a case with persistent dengue virus genome detectable in hematopoietic cells of a person with remote infection.

Methods: A patient with multiple myeloma in remission was prepared for peripheral blood stem cell (PBSC) transplantation. Plasma and G-CSF-stimulated, mobilized PBSCs were collected. Dengue-specific reverse transcription polymerase chain reaction (RT-PCR) was performed in both pre- and post-stimulated blood specimens. Anti-dengue antibodies by ELISA and by neutralization assay were measured before and after the stem cell mobilization.

Results: The viral genome was detected only in the PBSC of the post-G-CSF-stimulated specimens. Anti-dengue antibodies were negative and positive, by ELISA and neutralization assays, respectively, both before and after stem cell mobilization.

Conclusion: Our findings reveal a persistent infection. Whether and how this strain may interact with subsequent serotype(s) remains to be elucidated.

Keywords: G-CSF; dengue; hematopoietic; pathogenesis; persistence; serotype; stem cell person with remote infection.

Publication types

Case Reports