The complete chloroplast genome of Pulsatilla chinensis f. alba D. K. Zang (Ranunculaceae, Pulsatilla Miller)

Che Bian; Yan-Ping Xing; He-Fei Xue; Wen-Juan Hou; Yan-Yun Yang; Liang Xu; Ting-Guo Kang

doi:10.1080/23802359.2023.2294894

The complete chloroplast genome of Pulsatilla chinensis f. alba D. K. Zang (Ranunculaceae, Pulsatilla Miller)

Mitochondrial DNA B Resour. 2024 Jan 31;9(2):233-236. doi: 10.1080/23802359.2023.2294894. eCollection 2024.

Authors

Che Bian¹, Yan-Ping Xing¹, He-Fei Xue¹, Wen-Juan Hou¹, Yan-Yun Yang¹, Liang Xu¹, Ting-Guo Kang¹

Affiliation

¹ School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, China.

Abstract

Pulsatilla chinensis f. alba D. K. Zang 1993 is a forma of Pulsatilla chinensis (Bge.) Regel, the root of P. chinensis is traditional Chinese medicine called Pulsatillae radix. The biggest difference between P. chinensis f. alba and P. chinensis is that P. chinensis f. alba sepals is white. The complete chloroplast genome of P. chinensis f. alba was sequenced using the Illumina NovaSeq platform for the first time. The lengths of the genome, large single-copy (LSC), small single-copy (SSC), two inverted repeats (IRs), and GC content were 163,654 bp, 82,355 bp, 19,069 bp, 31,115 bp, and 37.2%, respectively. It had 134 genes, including 90 protein-coding genes, 36 tRNA genes, and eight rRNA genes. The maximum-likelihood tree indicated that P. chinensis f. alba had a closer relationship with P. chinensis. This study would provide a theoretical basis for the further study of Pulsatilla plants genetics phylogenetic research.

Keywords: Complete chloroplast genome; Pulsatilla chinensis f. alba D. K. Zang; Ranunculaceae; phylogenetic tree.

Grants and funding

This research was funded by Liaoning BaiQianWan Talents Program [No.2021A039], 2019 Liaoning Provincial Department of Education Scientific Research Project [L201942], National Key Research and Development in the 13th Five-Year Plan [2018YFC1708200], Major Special Fund for Science and Technology of Inner Mongolia Autonomous Region [2019ZD004], Natural Science Fund Project of Liaoning Province [2020-MS-224]. National Natural Science Foundation of China [82373999]. The ability establishment of sustainable use for valuable Chinese medicine resources [2060302].