Evaluation of Oxygen Consumption Rates In Situ

Anqi Li; Yuan Qin; Ying Zhang; Xiaoqun Zhen; Guohua Gong

doi:10.1007/978-1-0716-3633-6_16

Evaluation of Oxygen Consumption Rates In Situ

Methods Mol Biol. 2024:2755:215-226. doi: 10.1007/978-1-0716-3633-6_16.

Authors

Anqi Li^{1

2}, Yuan Qin³, Ying Zhang², Xiaoqun Zhen^{1

4}, Guohua Gong^{1

2}

Affiliations

¹ Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China.
² Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China.
³ Department of Pharmacy, Shanghai East Hospital, Tongji University, Shanghai, China.
⁴ Department of Clinical Laboratory, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.

PMID: 38319581
DOI: 10.1007/978-1-0716-3633-6_16

Abstract

An analysis of the mitochondrial respiration function represented by the oxygen consumption rate is necessary to assess mitochondrial bioenergetics and redox function. This protocol describes two alternative techniques to evaluate mitochondrial respiration function in situ: (1) measure oxygen consumption rates via an electrode; (2) measure oxygen consumption rates via a seahorse instrument. These in situ approaches provide more physiological access to mitochondria to evaluate mitochondrial respiration function in a relatively integrated cellular system.

Keywords: ATP; Mitochondria; Mitochondrial stress test; Oxidative phosphorylation; Oxygen consumption.

MeSH terms

Electrodes
Mitochondria
Oxygen Consumption*
Respiration*
Respiratory Function Tests