MXenes have attracted the attention of many researchers as one of the latest two-dimensional (2D) materials over the last decade. Their great potential for biosensing has also been fully exploited after the discovery of their unique properties such as superior optical properties, excellent hydrophilicity, good thermal stability, excellent mechanical property, high electrical conductivity, biocompatibility, large surface area, and ease of surface functionalization. In the MXene-based luminescence sensing strategy, MXenes typically appear in the form of nanosheets, quantum dots and modified MXene nanocomposites, and they are utilized as different sensing platforms or as a luminescence source. In this review, we focused on the MXene-based luminescence sensing strategies, including fluorescence, electrochemiluminescence and chemiluminescence sensors and the comparison of their performance. Finally, the perspectives of the MXene-based luminescence sensors are discussed.