Measurement of Autophagic Activity in Cancer Cells with Flow Cytometric Analysis Using Cyto-ID Staining

Merve Şansaçar; Emel Başak Gencer Akçok

doi:10.1007/7651_2024_526

Measurement of Autophagic Activity in Cancer Cells with Flow Cytometric Analysis Using Cyto-ID Staining

Methods Mol Biol. 2024 Mar 7. doi: 10.1007/7651_2024_526. Online ahead of print.

Authors

Merve Şansaçar^{1

2}, Emel Başak Gencer Akçok³

Affiliations

¹ Graduate School of Engineering and Science, Department of Bioengineering, Abdullah Gul University, Kayseri, Turkey.
² Faculty of Engineering, Department of Genetic and Bioengineering, Alanya Alaaddin Keykubat University, Alanya, Antalya, Turkey.
³ Faculty of Life and Natural Sciences, Department of Molecular Biology and Genetics, Abdullah Gul University, Kayseri, Turkey. emelbasak.gencerakcok@agu.edu.tr.

PMID: 38446407
DOI: 10.1007/7651_2024_526

Abstract

Autophagy is an evolutionarily conserved process providing the energy that cells need to survive, especially in stress situations, through catabolic processes. Considering the dual role of autophagy in cancer cells depending on the cellular context, it is crucial to comprehend the effect of drug candidates put forward to prevent cancer through the autophagy pathway. The CYTO-ID^® Autophagy Detection Kit allows a rapid, specific and quantitative measurement of autophagic activity at the cellular level using a 488 nm-excitable green fluorescent detection reagent via flow cytometer. In this chapter, we present the CYTO-ID^® Autophagy Detection method with a stepwise protocol to monitor the autophagy flux after the application of any compound to suspension cancer cell lines with flow cytometric analysis.

Keywords: Autophagic compartments; Autophagic flux; Autophagosome; Autophagy; Cancer; Cyto-ID; Flow cytometry.