Monitoring ADSC Metabolism Using the Seahorse Analyzer

Daniel Rittenhouse; Kevin J Zwezdaryk

doi:10.1007/978-1-0716-3762-3_24

Monitoring ADSC Metabolism Using the Seahorse Analyzer

Methods Mol Biol. 2024:2783:335-347. doi: 10.1007/978-1-0716-3762-3_24.

Authors

Daniel Rittenhouse¹, Kevin J Zwezdaryk^{2

3

4}

Affiliations

¹ Department of Microbiology & Immunology, Tulane University School of Medicine, New Orleans, LA, USA.
² Department of Microbiology & Immunology, Tulane University School of Medicine, New Orleans, LA, USA. kzwezdar@tulane.edu.
³ Tulane Center for Aging, Tulane University School of Medicine, New Orleans, LA, USA. kzwezdar@tulane.edu.
⁴ Tulane Brain Institute, Tulane University School of Medicine, New Orleans, LA, USA. kzwezdar@tulane.edu.

PMID: 38478245
DOI: 10.1007/978-1-0716-3762-3_24

Abstract

Bioenergetic and biosynthetic processes are key indicators regulating adipose-derived stromal/stem cell (ADSC) function, health, and differentiation. A common method used to metabolically profile cells is the Seahorse XF Analyzer. This live-cell assay can be used to define key metabolic pathways, including glycolysis and oxidative phosphorylation. Here, we share optimized protocols to characterize metabolism of ADSCs under basal conditions and provide insight into further assays defining metabolic changes and/or dependency during ADSC differentiation.

Keywords: ADSC; Differentiation; ETC; Glycolysis; Metabolism; Mitochondria; OXPHOS; Seahorse Analyzer.

MeSH terms

Adipocytes
Adipose Tissue / metabolism
Animals
Energy Metabolism
Glycolysis
Oxidative Phosphorylation
Smegmamorpha* / metabolism