Visualization of Fagopyrum esculentum and Fagopyrum tataricum Chromosomes and Micronuclei

Reneé Pérez-Pérez; Jolanta Kwasniewska

doi:10.1007/978-1-0716-3794-4_8

Visualization of Fagopyrum esculentum and Fagopyrum tataricum Chromosomes and Micronuclei

Methods Mol Biol. 2024:2791:81-87. doi: 10.1007/978-1-0716-3794-4_8.

Authors

Reneé Pérez-Pérez¹, Jolanta Kwasniewska²

Affiliations

¹ Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Katowice, Poland.
² Plant Cytogenetics and Molecular Biology Group, Faculty of Natural Sciences, University of Silesia in Katowice, Katowice, Poland. jolanta.kwasniewska@us.edu.pl.

PMID: 38532094
DOI: 10.1007/978-1-0716-3794-4_8

Abstract

This chapter presents the squash chromosome preparation technique for Fagopyrum esculentum and F. tataricum, using the root tips as the source of the material. Using an optimized version of this method, the chromosomes are free of cytoplasmic debris and are spread evenly on the glass slide. What comes of it is the possibility to make observations of the chromosome number and structure at the metaphase stage. This technique's modified version allows micronuclei analysis in interphase cells of buckwheats.

Keywords: Buckwheat; Chromosomes; Enzyme cocktail; Fagopyrum; Micronuclei; Mitosis; Squashed slides.

MeSH terms

Chromosomes
Fagopyrum* / chemistry
Fagopyrum* / genetics