Effect of MSX1 on the cellular function of cardiomyocytes

Huang Linhuan; Zhong Liangying; Lin Shaobin; Zhu Caixia; Cai Danlei; Huang Siqi; Hong Peiming; Kong Shu; Xie Yingjun; Luo Yanmin

doi:10.1016/j.gene.2024.148419

Effect of MSX1 on the cellular function of cardiomyocytes

Gene. 2024 Jul 20:916:148419. doi: 10.1016/j.gene.2024.148419. Epub 2024 Mar 29.

Authors

Huang Linhuan¹, Zhong Liangying², Lin Shaobin³, Zhu Caixia⁴, Cai Danlei⁵, Huang Siqi⁶, Hong Peiming⁷, Kong Shu⁸, Xie Yingjun⁹, Luo Yanmin¹⁰

Affiliations

¹ Department of Obstetrics and Gynecology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510100, Guangdong Province, PR China. Electronic address: hlhuan@mail.sysu.edu.cn.
² Department of Clinical Laboratory, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510100, Guangdong Province, PR China. Electronic address: zhongly6@mail.sysu.edu.cn.
³ Department of Obstetrics and Gynecology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510100, Guangdong Province, PR China. Electronic address: linshb7@mail.sysu.edu.cn.
⁴ Department of Obstetrics and Gynecology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510100, Guangdong Province, PR China. Electronic address: zhucx3@mailsysu.edu.cn.
⁵ Department of Ultrasound, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510100, Guangdong Province, PR China. Electronic address: caidanl@mail.sysu.edu.cn.
⁶ Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, Guangdong Province, PR China. Electronic address: huangsiqi226@163.com.
⁷ Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, Guangdong Province, PR China. Electronic address: 2357246063@qq.com.
⁸ Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, Guangdong Province, PR China. Electronic address: 553179851@qq.com.
⁹ Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, Guangdong Province, PR China. Electronic address: xieyjun@mail2.sysu.edu.cn.
¹⁰ Department of Obstetrics and Gynecology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510100, Guangdong Province, PR China. Electronic address: luoyanm@mail.sysu.edu.cn.

PMID: 38556116
DOI: 10.1016/j.gene.2024.148419

Abstract

MSX1 (Muscle Segment Homeobox 1) has pleiotropic effects in various tissues, including cardiomyocytes, while the effect of MSX1 on cardiomyocyte cellular function was not well known. In this study, we used AC16 cell culture, real-time fluorescence quantitative PCR (qPCR), protein blotting (Western blot), flow cytometry apoptosis assay and lactate dehydrogenase (LDH) ELISA (Enzyme-Linked Immunosorbnent Assay) to investigate the effect of the MSX1 gene on cardiomyocyte function. The results showed that MSX1 plays a protective role against hypoxia of cardiomyocytes. However, further studies are required to fully understand the role of MSX1 in the regulation of LDH expression in different cell types and under different conditions.

Keywords: Cardiomyocytes; LDH; MSX1.

MeSH terms

Animals
Apoptosis* / genetics
Cell Hypoxia / genetics
Cell Line
Humans
L-Lactate Dehydrogenase / genetics
L-Lactate Dehydrogenase / metabolism
MSX1 Transcription Factor* / genetics
MSX1 Transcription Factor* / metabolism
Myocytes, Cardiac* / metabolism

Substances

MSX1 Transcription Factor
L-Lactate Dehydrogenase