Brain defects often lead to motor dysfunctions in humans. Drosophila melanogaster has been one of the most useful organisms in the study of neuronal biology due to its similarities with humans and has contributed to a more detailed understanding of the effects of genetic dysfunctions in the brain on behavior. We herein present modified protocols for the crawling assay with larvae and the climbing assay with adult flies that are simple to perform as well as a series of commands for ImageJ to automatically analyze data for the crawling assay.
Keywords: Automatic analysis; Climbing assay; Crawling assay; Drosophila larval and adult locomotor ability.
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.