Nematicidal glycosylated resorcylic acid lactones from the fungus Pochonia chlamydosporia PC-170 and their key biosynthetic genes

Zeyu Li; Ning Luo; Wenwen Zhang; Raja Asad Ali Khan; Jian Ling; Jianlong Zhao; Yuhong Yang; Zhenchuan Mao; Bingyan Xie; Ligang Zhou; Yan Li

doi:10.3389/fmicb.2024.1385255

Nematicidal glycosylated resorcylic acid lactones from the fungus Pochonia chlamydosporia PC-170 and their key biosynthetic genes

Front Microbiol. 2024 Apr 4:15:1385255. doi: 10.3389/fmicb.2024.1385255. eCollection 2024.

Authors

Zeyu Li^{1

2}, Ning Luo³, Wenwen Zhang¹, Raja Asad Ali Khan¹, Jian Ling¹, Jianlong Zhao¹, Yuhong Yang¹, Zhenchuan Mao¹, Bingyan Xie¹, Ligang Zhou², Yan Li¹

Affiliations

¹ State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
² Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, China.
³ Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou, China.

Abstract

Chemical study of the nematicidal biocontrol fungus Pochonia chlamydosporia PC-170 led to discovery of six resorcylic acid lactones (RALs), including three nematicidal glycosylated RALs, monocillin VI glycoside (1), colletogloeolactone A (2) and monocillin II glycoside (3), and three antibacterial non-glycosylated RALs, monocillin VI (4), monocillin IV (5) and monocillin II (6). The planar structure of the new compound monocillin VI glycoside (1) was elucidated using HRESIMS and NMR data, and its monosaccharide configuration was further determined through sugar hydrolysis experiment and GC-MS analysis method. Furthermore, their two biosynthetic-related PKS genes, pchE and pchI, were identified through the gene knockout experiment. The glycosylated RALs 1-3 exhibited nematicidal activity against Meloidogyne incognita, with LC₅₀ values of 94, 152 and 64 μg/mL, respectively, and thus had great potential in the development of new nematicidal natural products to control M. incognita in the future.

Keywords: Meloidogyne incognita; Pochonia chlamydosporia; RALs; biosynthetic gene; nematicidal activity.

Grants and funding

The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This study was financially supported by the grants from the National Key R&D Program of China (2022YFD1400700 and 2023YFD1400400) and the Agricultural Science and Technology Innovation Program of CAAS.