Benastatin K, a chlorinated benastatin-related antibiotic from Streptomyces sp. HGTA384

Teppei Kawahara; Kanako Saita; Rika Iwamoto; Mikiyo Wada

doi:10.1038/s41429-024-00727-1

Benastatin K, a chlorinated benastatin-related antibiotic from Streptomyces sp. HGTA384

J Antibiot (Tokyo). 2024 Apr 25. doi: 10.1038/s41429-024-00727-1. Online ahead of print.

Authors

Teppei Kawahara¹, Kanako Saita², Rika Iwamoto³, Mikiyo Wada⁴

Affiliations

¹ Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto, 862-0973, Japan. teppei@kumamoto-u.ac.jp.
² Kumamoto Industrial Research Institute, 3-11-38 Higashi-machi, Higashi-ku, Kumamoto, 862-0901, Japan.
³ School of Pharmacy, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto, 862-0973, Japan.
⁴ Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-ku, Kumamoto, 862-0973, Japan.

PMID: 38664572
DOI: 10.1038/s41429-024-00727-1

Abstract

Benastatin K (1), a new chlorinated benastatin derivative, was isolated from the culture broth of the actinomycete Streptomyces sp. HGTA384. The structure of 1 was determined on the basis of spectroscopic analysis, including 1D and 2D NMR, as well as HRESI-MS, UV and IR, and comparison with data reported in the literature. Compound 1 and benastatins A and B exhibited inhibitory activity against Micrococcus luteus (MIC 7.8, 31.3, and 3.9 μM, respectively), and IgE-mediated β-hexosaminidase release in RBL-2H3 cells with IC₅₀ values of 42, 79, and 19 μM, respectively.

Grants and funding

JP19K07135/MEXT | Japan Society for the Promotion of Science (JSPS)