MEN1 deficiency stabilizes PD-L1 and promotes tumor immune evasion of lung cancer

Abstract

Multiple Endocrine Neoplasia 1 gene (MEN1), which is known to be a tumor suppressor gene in lung tissues, encodes a 610 amino acid protein menin. Previous research has proven that MEN1 deficiency promotes the malignant progression of lung cancer. However, the biological role of this gene in the immune microenvironment of lung cancer remains unclear. In this study, we found that programmed cell death-ligand 1 (PD-L1) is upregulated in lung-specific Kras^G12D mutation-induced lung adenocarcinoma in mice, after Men1 deficiency. Simultaneously, CD8⁺ and CD3⁺ T cells are depleted, and their cytotoxic effects are suppressed. In vitro, PD-L1 is inhibited by the overexpression of menin. Mechanistically, we found that MEN1 inactivation promotes the deubiquitinating activity of COP9 signalosome subunit 5 (CSN5) and subsequently increases the level of PD-L1.

Keywords: MEN1; CSN5; PD‐L1; immune evasion; lung cancer.