Effective enrichment and separation of three flavonoids from Ohwia caudata (Thunberg) H. Ohashi using magnetic layered double hydroxide/ZIF-8 composites and pCEC

Tingfan Tang; Ziwei Xu; Ying Wang; Xuesong Li; Lijun Li; Hao Cheng; Yuhong Tian; Wenyi Huang; Jun Feng

doi:10.1016/j.jpba.2024.116161

Effective enrichment and separation of three flavonoids from Ohwia caudata (Thunberg) H. Ohashi using magnetic layered double hydroxide/ZIF-8 composites and pCEC

J Pharm Biomed Anal. 2024 Apr 18:245:116161. doi: 10.1016/j.jpba.2024.116161. Online ahead of print.

Authors

Tingfan Tang¹, Ziwei Xu², Ying Wang², Xuesong Li², Lijun Li¹, Hao Cheng¹, Yuhong Tian², Wenyi Huang³, Jun Feng⁴

Affiliations

¹ Guangxi Key Laboratory of Green Processing of Sugar Resources, Department of Medicine/College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou, Guangxi 545006, PR China; Provine and Ministry Co-sponsored Collaborative Innovation Center of Sugarcane and Sugar Industry, Nanning, Guangxi 530004, PR China.
² Guangxi Key Laboratory of Green Processing of Sugar Resources, Department of Medicine/College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou, Guangxi 545006, PR China.
³ Guangxi Key Laboratory of Green Processing of Sugar Resources, Department of Medicine/College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou, Guangxi 545006, PR China; Provine and Ministry Co-sponsored Collaborative Innovation Center of Sugarcane and Sugar Industry, Nanning, Guangxi 530004, PR China. Electronic address: hwylz18@163.com.
⁴ Guangxi Key Laboratory of Green Processing of Sugar Resources, Department of Medicine/College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou, Guangxi 545006, PR China. Electronic address: hxpfengjun@gxust.edu.cn.

PMID: 38714135
DOI: 10.1016/j.jpba.2024.116161

Abstract

In this study, Fe₃O₄@ZnCr-layered double hydroxide/zeolitic imidazolate frameworks-8 (MLDH/ZIF-8) magnetically functionalized composites were synthesized by co-precipitation and in situ growth based on the advantages of LDHs and ZIF-8 using Fe₃O₄ nanoparticles as a magnetic substrate to obtain adsorbents with excellent performance. Moreover, the composite was used for the efficient enrichment of flavonoids in Chinese herbal medicines. The internal structures and surface properties were characterized by SEM, Fourier transform infrared spectroscopy, X-ray diffraction and so on. MLDH/ZIF-8 exhibited a large specific surface area and good paramagnetic properties. The MLDH/ZIF-8 magnetic composite was used as a magnetic solid-phase extraction (MSPE) adsorbent, and a MLDH/ZIF-8 MSPE-pressurized capillary electrochromatography coupling method was developed for the separation and detection of flavonoids (luteolin, kaempferol and apigenin) in a sample of the Chinese herb Ohwia caudata (Thunberg) H. Ohashi. The relevant parameters affecting the extraction efficiency were optimized to determine the ideal conditions for MSPE. 5 mg of adsorbent in sample solution at pH 6, vortex extraction for 5 min, elution with 1.5 mL of ethyl acetate for 15 min. The method showed good linearity in the concentration range of 3-50 μg mL^-1 with correlation coefficients of 0.9934-0.9981, and displayed a relatively LODs of 0.07-0.09 μg mL^-1. The spiked recoveries of all analytes ranged from 84.5% to 122.0% with RSDs (n=3) between 4.5% and 7.7%. This method is straightforward and efficient, with promising potential in the separation and analysis of active ingredients in various Chinese herbal medicines.

Keywords: Flavonoids; Layered double hydroxides (LDH); Magnetic solid-phase extraction (MSPE); Pressurized capillary electrochromatography (pCEC); Zeolitic imidazolate frameworks-8 (ZIF-8).