Predictive Value of Serum microRNA-29b-3p in Recurrence of Atrial Fibrillation After Radiofrequency Catheter Ablation

Clin Interv Aging. 2024 May 3:19:715-725. doi: 10.2147/CIA.S450292. eCollection 2024.

Abstract

Objective: Atrial fibrillation (AF) is a common arrhythmia. This study explored serum miR-29b-3p expression in AF patients and its value in predicting AF recurrence after radiofrequency catheter ablation (RFCA).

Methods: Totally 100 AF patients who underwent RFCA were enrolled, with 100 individuals without AF as controls. Serum miR-29b-3p expression in participants was determined using RT-qPCR. The correlation between miR-29b-3p and atrial fibrosis markers (FGF-21/FGF-23) was assessed by Pearson analysis. The diagnostic efficacy of serum miR-29b-3p and FGF-21/FGF-23 in predicting AF recurrence after RFCA was analyzed by the receiver operating characteristic (ROC) curves. The Kaplan-Meier method was adopted to evaluate the effect of miR-29b-3p expression on the incidence of AF recurrence after RFCA. The independent risk factors for AF recurrence after RFCA were analyzed by logistic regression analysis.

Results: Serum miR-29b-3p was poorly expressed in AF patients. After RFCA, AF patients showed elevated serum miR-29b-3p expression. Serum miR-29b-3p expression in AF patients negatively correlated with serum FGF-21 and FGF-23 concentrations. The cut-off values of serum miR-29b-3p, FGF-21, and FGF-23 in identifying AF recurrence were 0.860 (sensitivity: 100.00%, specificity: 39.71%), 222.2 pg/mL (sensitivity: 96.88%, specificity: 32.35%) and 216.3 ng/mL (sensitivity: 53.13%, specificity: 70.59%), respectively. Patients with low miR-29b-3p expression had a significantly higher incidence of AF recurrence than patients with high miR-29b-3p expression. Serum miR-29b-3p expression was one of the independent risk factors for AF recurrence after RFCA.

Conclusion: Low miR-29b-3p expression in AF patients has certain predictive values and is one of the independent risk factors for AF recurrence after RFCA.

Keywords: atrial fibrillation; fibrosis marker; logistic regression analysis; miR-29b-3p; radiofrequency catheter ablation; recurrence.

MeSH terms

  • Aged
  • Atrial Fibrillation* / blood
  • Biomarkers / blood
  • Catheter Ablation*
  • Female
  • Fibroblast Growth Factor-23
  • Fibroblast Growth Factors / blood
  • Humans
  • Male
  • MicroRNAs* / blood
  • Middle Aged
  • Predictive Value of Tests
  • ROC Curve
  • Recurrence*
  • Risk Factors

Substances

  • MicroRNAs
  • MIRN29a microRNA, human
  • Fibroblast Growth Factor-23
  • FGF23 protein, human
  • Biomarkers
  • Fibroblast Growth Factors

Grants and funding

This work was partially supported by grants from The National Natural Science Foundation of China (82102744); Applied Basic Research Program of Liaoning Province (2022JH2/101500011); Livelihood Science and Technology Plan Joint Plan Project of Liaoning Province (2021JH2/10300098); Doctoral Start-up Foundation of Liaoning Province (2021-BS-029); China Post-Doctoral Science Foundation under Grant Number (2023M744306).