Investigating cultivation strategies for enhancing protein content in Auxenochlorella pyrenoidosa FACHB-5

Qun Wei; Ting Yuan; Zhuang Li; Dan Zhao; Canmei Wang; Gairen Yang; Wangwang Tang; Xiangmeng Ma

doi:10.1016/j.biortech.2024.130828

Investigating cultivation strategies for enhancing protein content in Auxenochlorella pyrenoidosa FACHB-5

Bioresour Technol. 2024 May 10:402:130828. doi: 10.1016/j.biortech.2024.130828. Online ahead of print.

Authors

Qun Wei¹, Ting Yuan², Zhuang Li², Dan Zhao², Canmei Wang², Gairen Yang³, Wangwang Tang⁴, Xiangmeng Ma⁵

Affiliations

¹ School of Resources, Environment and Materials, Guangxi University, No. 100 Daxue Road, Nanning, Guangxi 530004, China; Key Laboratory of Environmental Protection (Guangxi University), Education Department of Guangxi Zhuang Autonomous Region, Guangxi Nanning 530004, China; Guangxi Key Laboratory of Emerging Contaminants Monitoring, Early Warning and Environmental Health Risk Assessment.
² School of Resources, Environment and Materials, Guangxi University, No. 100 Daxue Road, Nanning, Guangxi 530004, China.
³ Forestry College of Guangxi University, Guangxi Key Laboratory of Forest Ecology and Conservation, Guangxi University, No. 100 Daxue Road, Nanning 530004, PR China.
⁴ College of Environmental Science and Engineering, Hunan University, Changsha 410082, China.
⁵ School of Resources, Environment and Materials, Guangxi University, No. 100 Daxue Road, Nanning, Guangxi 530004, China; Key Laboratory of Environmental Protection (Guangxi University), Education Department of Guangxi Zhuang Autonomous Region, Guangxi Nanning 530004, China; Guangxi Key Laboratory of Emerging Contaminants Monitoring, Early Warning and Environmental Health Risk Assessment. Electronic address: maxiangmeng@gxu.edu.cn.

PMID: 38734260
DOI: 10.1016/j.biortech.2024.130828

Abstract

This study investigated the influence of yeast extract addition, carbon source, and photoperiod on the growth dynamics of Auxenochlorella pyrenoidosa FACHB-5. Employing response surface methodology, the culture strategy was optimized, resulting in the following optimal conditions: yeast extract addition at 0.75 g L^-1, glucose concentration of 0.83 g L^-1, and a photoperiod set at Light: Dark = 18 h: 6 h. Under these conditions, the biomass reached 1.76 g L^-1 with a protein content of 750.00 g L^-1, containing 40 % of essential amino acids, representing a 1.52-fold increase. Proteomic analysis revealed that the targeted cultivation strategy up-regulated genes involved in microalgal protein synthesis. The combined effect of yeast extract and glucose enhanced both the glutamine synthetase-glutamate synthetase mechanism and the free amino acid content.

Keywords: Amino acids; Microalgal proteins; Proteomics; Response surface methodology.