IKVAV functionalized oriented PCL/Fe3O4 scaffolds for magnetically modulating DRG growth behavior

Yaqiong Liu; Hongxia Gao; Yuqing Shang; Shaolan Sun; Wenchao Guan; Tiantian Zheng; Linliang Wu; Meng Cong; Luzhong Zhang; Guicai Li

doi:10.1016/j.colsurfb.2024.113967

IKVAV functionalized oriented PCL/Fe₃O₄ scaffolds for magnetically modulating DRG growth behavior

Colloids Surf B Biointerfaces. 2024 May 16:239:113967. doi: 10.1016/j.colsurfb.2024.113967. Online ahead of print.

Authors

Affiliations

¹ Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, PR China; Guangdong Provincial Key Laboratory of Advanced Biomaterials, Southern University of Science and Technology, Shenzhen 518055, PR China.
² Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, PR China.
³ Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, PR China; The People's Hospital of Rugao, Affiliated Hospital of Nantong University, Nantong 226599, PR China.
⁴ Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, PR China; Guangdong Provincial Key Laboratory of Advanced Biomaterials, Southern University of Science and Technology, Shenzhen 518055, PR China. Electronic address: gcli1981@ntu.edu.cn.

PMID: 38761494
DOI: 10.1016/j.colsurfb.2024.113967

Abstract

The re-bridging of the deficient nerve is the main problem to be solved after the functional impairment of the peripheral nerve. In this study, a directionally aligned polycaprolactone/triiron tetraoxide (PCL/Fe₃O₄) fiber scaffolds were firstly prepared by electrospinning technique, and further then grafted with IKVAV peptide for regulating DRG growth and axon extension in peripheral nerve regeneration. The results showed that oriented aligned magnetic PCL/Fe₃O₄ composite scaffolds were successfully prepared by electrospinning technique and possessed good mechanical properties and magnetic responsiveness. The PCL/Fe₃O₄ scaffolds containing different Fe₃O₄ concentrations were free of cytotoxicity, indicating the good biocompatibility and low cytotoxicity of the scaffolds. The IKVAV-functionalized PCL/Fe₃O₄ scaffolds were able to guide and promote the directional extension of axons, the application of external magnetic field and the grafting of IKVAV peptides significantly further promoted the growth of DRGs and axons. The ELISA test results showed that the AP-10 F group scaffolds promoted the secretion of nerve growth factor (NGF) from DRG under a static magnetic field (SMF), thus promoting the growth and extension of axons. Importantly, the IKVAV-functionalized PCL/Fe₃O₄ scaffolds could significantly up-regulate the expression of Cntn2, PCNA, Sox10 and Isca1 genes related to adhesion, proliferation and magnetic receptor function under the stimulation of SMF. Therefore, IKVAV-functionalized PCL/Fe₃O₄ composite oriented scaffolds have potential applications in neural tissue engineering.

Keywords: Axon extension; Electrospinning; Magnetic nanoparticles; Oriented scaffolds; Polycaprolactone.