Partial purification and characterisation of a 31 kDalton putative enkephalin precursor from guinea pig striatum

K M Metters; A Beaumont; J Rossier; J Hughes

doi:10.1016/0143-4179(85)90065-4

Partial purification and characterisation of a 31 kDalton putative enkephalin precursor from guinea pig striatum

Neuropeptides. 1985 Feb;5(4-6):505-8. doi: 10.1016/0143-4179(85)90065-4.

Authors

K M Metters, A Beaumont, J Rossier, J Hughes

PMID: 3889697
DOI: 10.1016/0143-4179(85)90065-4

Abstract

The presence of several putative precursors for Met-enkephalin (ME) and Leu-enkephalin (LE) has been demonstrated in guinea pig striatal extracts. The major high molecular weight (Mr) species (31000) contained 9% of the total ME-immunoreactivity (IR) after digest. This species has been partially purified and characterized using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and nitrocellulose blotting techniques as well as highly specific radioimmunoassay (RIA) for ME, LE and ME-Arg6-Phe7.

MeSH terms

Animals
Carboxypeptidase B
Carboxypeptidases
Catalysis
Corpus Striatum / analysis*
Electrophoresis, Polyacrylamide Gel
Endopeptidases
Enkephalins / isolation & purification*
Guinea Pigs
Male
Metalloendopeptidases*
Protein Precursors / isolation & purification*
Trypsin

Substances

Enkephalins
Protein Precursors
Carboxypeptidases
Endopeptidases
Carboxypeptidase B
Trypsin
Metalloendopeptidases
auR protein, Staphylococcus aureus