Identification of estradiol in the ovaries of the silkworm, Bombyx mori

E Ohnishi; M Ogiso; K Wakabayashi; Y Fujimoto; N Ikekawa

doi:10.1016/0016-6480(85)90289-8

Identification of estradiol in the ovaries of the silkworm, Bombyx mori

Gen Comp Endocrinol. 1985 Oct;60(1):35-8. doi: 10.1016/0016-6480(85)90289-8.

Authors

E Ohnishi, M Ogiso, K Wakabayashi, Y Fujimoto, N Ikekawa

PMID: 4054586
DOI: 10.1016/0016-6480(85)90289-8

Abstract

Estradiol was extracted and partially purified from the ovaries of the silkworm, Bombyx mori. Identification of estradiol was done by use of radioimmunoassay (RIA) and by gas chromatography-mass spectrometry (GC-MS) after derivatization into the ethyldimethylsilyl derivative. Concentration of estradiol in the ovaries was estimated to be 176 pg/g (RIA) and 63 pg/g (GC-MS).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bombyx / analysis*
Estradiol / analysis*
Female
Gas Chromatography-Mass Spectrometry
Ovary / analysis
Radioimmunoassay

Substances

Estradiol