Effect of peptone on Azotobacter morphology

J Bacteriol. 1972 Jul;111(1):260-6. doi: 10.1128/jb.111.1.260-266.1972.

Abstract

Pleomorphism in cultures of Azotobacter vinelandii was induced by addition of Difco peptone to the growth medium. Under the conditions of the study, 5% peptone gave rise to transient forms described as "fungoid cells" which were osmotically fragile. After some 48 hr of culture, they became osmotically stable and resumed a more typical morphology. It was shown that the pleomorphism-inducing principle in peptone was glycine.

MeSH terms

  • Amino Acids / analysis
  • Autoanalysis
  • Azotobacter / cytology*
  • Azotobacter / drug effects
  • Azotobacter / growth & development
  • Cell Wall / drug effects
  • Chromatography
  • Culture Media
  • Glycine / pharmacology
  • Microscopy, Electron
  • Microscopy, Phase-Contrast
  • Molecular Weight
  • Osmosis
  • Osmotic Fragility
  • Peptones / analysis
  • Peptones / pharmacology*
  • Ultrafiltration

Substances

  • Amino Acids
  • Culture Media
  • Peptones
  • Glycine