Interaction between heparin and human pregnancy-associated plasma protein A (PAPP-A): a simple purification procedure

Anal Biochem. 1983 May;131(1):18-24. doi: 10.1016/0003-2697(83)90130-6.

Abstract

Human pregnancy-associated plasma protein A (PAPP-A) binds to heparin-Sepharose. This affinity chromatography preceded by molecular sieve chromatography provides a simple two-step purification procedure of PAPP-A from late pregnancy plasma. One hundred percent of the applied PAPP-A was recovered, with more than 40% being electrophoretically homogeneous after the two procedures. The remaining PAPP-A could be purified by negative affinity chromatography on anti-total human serum immobilized on agarose.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Affinity
  • Chromatography, Gel
  • Female
  • Heparin*
  • Humans
  • Immunoelectrophoresis
  • Pregnancy
  • Pregnancy Proteins / isolation & purification*
  • Pregnancy-Associated Plasma Protein-A / isolation & purification*
  • Sepharose

Substances

  • Pregnancy Proteins
  • Heparin
  • Sepharose
  • Pregnancy-Associated Plasma Protein-A