Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes

Anal Biochem. 1980 Nov 15;109(1):76-86. doi: 10.1016/0003-2697(80)90013-5.
No abstract available

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetone
  • DNA Topoisomerases, Type I / isolation & purification*
  • DNA-Directed RNA Polymerases / isolation & purification*
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli / enzymology
  • Microchemistry
  • Molecular Weight
  • Plants / enzymology
  • Proteins / isolation & purification*
  • Sigma Factor / isolation & purification*
  • Sodium Dodecyl Sulfate
  • Transcription Factors / isolation & purification*

Substances

  • Proteins
  • Sigma Factor
  • Transcription Factors
  • Acetone
  • Sodium Dodecyl Sulfate
  • DNA-Directed RNA Polymerases
  • DNA Topoisomerases, Type I