Abstract
Viable human and murine lymphoblasts, and normal human tissue extracts, converted the thioether nucleosides 5'-methylthioadenosine (MeSAdo) and 5'-methylthioinosine (MeSIno) to methionine. Both MeSAdo and MeSIno, but not homocysteine, supported the short-term growth of human or murine lymphoblasts in methionine deficient medium. However, MeSAdo at concentrations greater than 25 microM inhibited cell growth. MeSIno was non-toxic at concentrations up to 200 microM, and supported the long-term growth of lymphoblasts in methionine-free medium.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine / analogs & derivatives*
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Adenosine / metabolism
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Adenosine / pharmacology
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Animals
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B-Lymphocytes / metabolism*
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Cell Division / drug effects*
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Cell Line
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Deoxyadenosines*
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Humans
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Inosine / analogs & derivatives*
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Leukemia L1210 / metabolism*
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Methionine / metabolism*
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Methylthioinosine / analogs & derivatives*
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Methylthioinosine / metabolism
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Methylthioinosine / pharmacology
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Mice
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Thionucleosides / metabolism
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Thionucleosides / pharmacology*
Substances
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Deoxyadenosines
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Thionucleosides
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5'-methylthioinosine
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Methylthioinosine
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Inosine
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5'-methylthioadenosine
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Methionine
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Adenosine