1. An enzyme which catalyzes the NAD(P)H-linked reversible reduction of uncharged vicinal dicarbonyls and alpha-hydroxycarbonyls to L-(+)-glycols has been purified from hen's muscle. This enzyme has not been previously described. 2. According to the rules of the I.U.P.A.C.-I.U.B. Enzymes Commission, the systematic name of L-(+)-glycol:NAD(P) oxidoreductase and the trivial name of L-glycol dehydrogenase are proposed for the enzyme. 3. Three forms of this enzyme differing in pI have been isolated; two forms, which together represent about 90% of total recovered activity, and electrophoretically pure. 4. Molecular weight, pH profiles and affinity for substrates are also described.