Determination of adeno-associated virus Rep68 and Rep78 binding sites by random sequence oligonucleotide selection

J Virol. 1995 Nov;69(11):7334-8. doi: 10.1128/JVI.69.11.7334-7338.1995.

Abstract

To further define the canonical binding site for the P5-promoted Rep proteins of the adeno-associated virus, a modified random oligonucleotide selection procedure was performed, using purified recombinant Rep protein. These results may explain the effects of Rep on cellular gene expression.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Binding Sites
  • Consensus Sequence
  • Conserved Sequence
  • DNA Primers
  • DNA, Viral / chemistry
  • DNA, Viral / metabolism
  • DNA-Binding Proteins / metabolism*
  • Dependovirus / metabolism*
  • Molecular Sequence Data
  • Oligonucleotide Probes / chemistry
  • Oligonucleotide Probes / metabolism*
  • Random Allocation
  • Recombinant Proteins / metabolism
  • Substrate Specificity
  • Viral Proteins / metabolism*

Substances

  • DNA Primers
  • DNA, Viral
  • DNA-Binding Proteins
  • Oligonucleotide Probes
  • Recombinant Proteins
  • Viral Proteins
  • rep proteins, Adeno-associated virus 2