Abstract
In response to specific extracellular signals, intracellular cyclic AMP levels increase, leading to a variety of responses including the alteration of transcription of many eukaryotic genes. This transcriptional effect is frequently mediated through the cyclic AMP-response element (CRE) motif T(T/G)ACGTCA. Using an expression screening approach we have cloned a yeast gene, MSN2, that encodes a 78 kDa protein that recognizes this consensus CRE motif. Phosphorylation of the MSN2 protein by the catalytic subunit of protein kinase A stimulates DNA binding in vitro. Two putative Cys2His2-type zinc fingers present in the C-terminal 79 amino acids of the MSN2 protein are sufficient to confer CRE-binding specificity. Therefore, MSN2 represents a novel CRE-binding protein distinct from the multiple previously characterized basic region-leucine zipper repeat CRE-binding proteins.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacteriophage lambda / genetics
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Cloning, Molecular*
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Cyclic AMP Response Element-Binding Protein / genetics*
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Cyclic AMP Response Element-Binding Protein / metabolism
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Cyclic AMP-Dependent Protein Kinases / metabolism
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DNA / metabolism
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Fungal Proteins / genetics*
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Fungal Proteins / metabolism
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Gene Expression / genetics
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Isopropyl Thiogalactoside / metabolism
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Phosphorylation
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Restriction Mapping
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Saccharomyces cerevisiae Proteins
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Transcription Factors*
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Yeasts / chemistry*
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Yeasts / genetics
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Zinc Fingers / genetics*
Substances
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Cyclic AMP Response Element-Binding Protein
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DNA-Binding Proteins
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Fungal Proteins
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MSN2 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Isopropyl Thiogalactoside
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DNA
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Cyclic AMP-Dependent Protein Kinases