Abstract
We have cloned the gene that encodes subunit 4 of the T. brucei cytochrome-c reductase complex and a fragment of the C. fasciculata subunit 4 cDNA and have shown that subunit 4 is the Rieske iron-sulfur protein. The cleaved presequences of the trypanosomatid iron-sulfur proteins resemble conventional mitochondrial targeting presequences but are smaller than other eukaryotic iron-sulfur protein signal peptides.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Biological Transport
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Cell Compartmentation
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Crithidia fasciculata / metabolism
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DNA Primers / chemistry
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Electron Transport Complex III*
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Iron-Sulfur Proteins / chemistry
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Iron-Sulfur Proteins / metabolism*
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Mitochondria / metabolism*
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Molecular Sequence Data
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NADH Dehydrogenase / chemistry
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NADH Dehydrogenase / metabolism*
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Protein Precursors / metabolism
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Protein Processing, Post-Translational
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Sequence Alignment
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Sequence Homology, Amino Acid
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Trypanosoma brucei brucei / metabolism
Substances
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DNA Primers
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Iron-Sulfur Proteins
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Protein Precursors
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Rieske iron-sulfur protein
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NADH Dehydrogenase
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Electron Transport Complex III
Associated data
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GENBANK/U28865
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GENBANK/U28866