Abstract
The effect of glutamate receptor agonists (NMDA, kainate, quisqualate and AMPA) on the [Ca2+]i and on [3H]GABA release was studied in cultured chick embryonic retinal cells. The release of [3H]GABA evoked by NMDA, in the absence of Ca2+, was prevented by the NMDA receptor antagonist (+)-methyl-10,11-dihydro-5H-dibenzo[a,d]-cyclohepten-5,10-imine (MK-801), and that produced by kainate and quisqualate was prevented by 6-cyano-7-nitroquinoxaline-2,3-dioxine (CNQX). All the agonists tested increased the [Ca2+]i, and when the GABA transporter was blocked by 1-(2-(((diphenyl-methylene)amino)oxy)ethyl)-1,2,5,6-tetrahydro-3- pyridine-carboxylic acid (NNC-711), NMDA, AMPA or quisqualate, but not kainate, did not induce [3H]GABA release unless Ca2+ (1 mM) was present, showing that exocytotic release of [3H]GABA occurs in retinal cells under these conditions.
MeSH terms
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6-Cyano-7-nitroquinoxaline-2,3-dione / pharmacology
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Animals
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Calcium / pharmacology*
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Cells, Cultured
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Chick Embryo
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Dizocilpine Maleate / pharmacology
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Excitatory Amino Acid Agonists / pharmacology*
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GABA Antagonists / pharmacology
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Kainic Acid / pharmacology
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N-Methylaspartate / pharmacology
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Nicotinic Acids / pharmacology
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Nipecotic Acids*
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Oximes / pharmacology
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Quisqualic Acid / pharmacology
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Retina / drug effects*
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Retina / embryology
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Retina / metabolism
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Tritium
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alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / pharmacology
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gamma-Aminobutyric Acid / metabolism*
Substances
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Excitatory Amino Acid Agonists
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GABA Antagonists
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Nicotinic Acids
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Nipecotic Acids
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Oximes
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Tritium
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NNC 711
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gamma-Aminobutyric Acid
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N-Methylaspartate
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Dizocilpine Maleate
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6-Cyano-7-nitroquinoxaline-2,3-dione
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alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
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Quisqualic Acid
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Kainic Acid
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Calcium