Ultraviolet B irradiation increases endothelin-1 and endothelin receptor expression in cultured human keratinocytes

R Tsuboi; C Sato; Y Oshita; H Hama; T Sakurai; K Goto; H Ogawa

doi:10.1016/0014-5793(95)00912-s

Ultraviolet B irradiation increases endothelin-1 and endothelin receptor expression in cultured human keratinocytes

FEBS Lett. 1995 Sep 4;371(2):188-90. doi: 10.1016/0014-5793(95)00912-s.

Authors

R Tsuboi¹, C Sato, Y Oshita, H Hama, T Sakurai, K Goto, H Ogawa

Affiliation

¹ Department of Dermatology, Juntendo University School of Medicine, Tokyo, Japan.

PMID: 7672125
DOI: 10.1016/0014-5793(95)00912-s

Abstract

The effect of ultraviolet B (UVB) irradiation on endothelin-1 (ET-1) and ET receptor expression was examined using cultured normal human keratinocytes. Keratinocytes secreted ET-1 in the medium at a level of 2.1 pg/day/10(5) cells. UVB irradiation up to 10 mJ/cm2 increased ET-1 secretion 3-fold, and potentiated expression of mRNA for ET-1. Both ETA and ETB receptor mRNAs were detected in keratinocytes, and their expression was up-regulated by 5 mJ/cm2 UVB irradiation.

MeSH terms

Base Sequence
Blotting, Northern
Cells, Cultured
Electrophoresis, Agar Gel
Endothelins / genetics*
Endothelins / metabolism
Gene Expression / radiation effects*
Humans
Keratinocytes / metabolism*
Molecular Sequence Data
RNA, Messenger / analysis
Receptors, Endothelin / genetics*
Ultraviolet Rays*

Substances

Endothelins
RNA, Messenger
Receptors, Endothelin