Abstract
Regulated Ca(2+)-dependent release of transmitters from synaptic vesicles is an important characteristic of chemical neurotransmission. Synaptotagmins are abundant synaptic vesicle transmembrane proteins that probably function as Ca2+ sensors. Molecular cloning has identified four different synaptotagmin isoforms in mammals. We report here the cloning and sequencing of a novel isoform of 386 amino acids. Synaptotagmin V is 54% identical in sequence to synaptotagmin I and possesses all the domains that characterise this multigene family. It is expressed at high levels in rat brain, but not in spinal cord or a number of peripheral non-neuronal tissues.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Brain / metabolism*
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Calcium-Binding Proteins*
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Cloning, Molecular
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DNA Primers
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DNA, Complementary
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Gene Expression*
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Gene Library
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Mammals
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Membrane Glycoproteins / biosynthesis*
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Membrane Glycoproteins / chemistry
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Molecular Sequence Data
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Nerve Tissue Proteins / biosynthesis*
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Nerve Tissue Proteins / chemistry
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Polymerase Chain Reaction
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RNA, Messenger / biosynthesis
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RNA, Messenger / isolation & purification
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Rats
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Rats, Sprague-Dawley
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Sequence Homology, Amino Acid
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Synaptic Vesicles / metabolism
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Synaptotagmin I
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Synaptotagmins
Substances
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Calcium-Binding Proteins
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DNA Primers
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DNA, Complementary
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Membrane Glycoproteins
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Nerve Tissue Proteins
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RNA, Messenger
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Recombinant Proteins
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Synaptotagmin I
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Syt1 protein, rat
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Syt5 protein, rat
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Synaptotagmins