Self assembly of covalently anchored phospholipid supported membranes by use of DODA-Suc-NHS-lipids

Biochim Biophys Acta. 1994 Dec 30;1196(2):227-30. doi: 10.1016/0005-2736(94)00218-5.

Abstract

We present a novel preparation method for the self assembly of covalently anchored phospholipid supported membranes. The surface is gold covered by cysteamine. Vesicles containing DMPC and activated DODA-Suc-NHS-lipids assembled on this surface. The whole self-assembly process is monitored conveniently by Near Infrared Surface Plasmon Resonance (NIR-SPR). Comparing the data to those obtained by Ca(2+)-mediated vesicle fusion, confirmed this interpretation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Dimyristoylphosphatidylcholine
  • Lipid Bilayers / chemistry*
  • Membranes / chemistry
  • Phosphatidylglycerols
  • Phospholipids / chemistry*
  • Succinimides*

Substances

  • Lipid Bilayers
  • N-((hydroxysuccinimidyl)-N-succinyl)dioctadecylamine
  • Phosphatidylglycerols
  • Phospholipids
  • Succinimides
  • dimyristoylphosphatidylglycerol
  • Dimyristoylphosphatidylcholine