Abstract
Transgenic Arabidopsis plants expressing a luciferase gene fused to a circadian-regulated promoter exhibited robust rhythms in bioluminescence. The cyclic luminescence has a 24.7-hour period in white light but 30- to 36-hour periods under constant darkness. Either red or blue light shortened the period of the wild type to 25 hours. A phytochrome-deficient mutation lengthened the period in continuous red light but had little effect in continuous blue light, whereas seedlings carrying mutations that activate light-dependent pathways in darkness maintained shorter periods in constant darkness. These results suggest that both phytochrome- and blue light-responsive photoreceptor pathways control the period of the circadian clock.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Arabidopsis / genetics
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Arabidopsis / physiology*
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Arabidopsis Proteins*
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Biological Clocks* / genetics
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Carrier Proteins / genetics
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Circadian Rhythm* / genetics
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Darkness
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Gene Expression Regulation, Plant
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Genes, Plant*
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Light*
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Light-Harvesting Protein Complexes
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Luciferases / genetics
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Luminescence
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Photosynthetic Reaction Center Complex Proteins*
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Photosystem II Protein Complex*
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Plant Proteins*
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Plants, Genetically Modified
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Recombinant Fusion Proteins
Substances
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Arabidopsis Proteins
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CAB1 protein, Arabidopsis
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Carrier Proteins
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Light-Harvesting Protein Complexes
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Photosynthetic Reaction Center Complex Proteins
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Photosystem II Protein Complex
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Plant Proteins
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Recombinant Fusion Proteins
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Luciferases