Adjuvant Quil A improves protection in mice and enhances opsonic capacity of antisera induced by pneumococcal polysaccharide conjugate vaccines

E A DeVelasco; H A Dekker; P Antal; K P Jalink; J A van Strijp; A F Verheul; J Verhoef; H Snippe

doi:10.1016/0264-410x(94)90151-1

Adjuvant Quil A improves protection in mice and enhances opsonic capacity of antisera induced by pneumococcal polysaccharide conjugate vaccines

Vaccine. 1994 Nov;12(15):1419-22. doi: 10.1016/0264-410x(94)90151-1.

Authors

E A DeVelasco¹, H A Dekker, P Antal, K P Jalink, J A van Strijp, A F Verheul, J Verhoef, H Snippe

Affiliation

¹ Eijkman-Winkler Institute of Medical Microbiology, Utrecht University, The Netherlands.

PMID: 7887020
DOI: 10.1016/0264-410x(94)90151-1

Abstract

The adjuvant effect of Quil A on the primary antibody response of mice to pneumococcal capsular polysaccharide conjugates was examined. Quil A increased the anti-capsular polysaccharide antibody titres, the protection against Streptococcus pneumoniae, and the opsonic capacity of the antibodies as measured in a newly developed in vitro phagocytosis assay, using the mouse macrophage cell line J774.

MeSH terms

Adjuvants, Immunologic / pharmacology*
Animals
Bacterial Vaccines / immunology*
Cell Line
Immune Sera / immunology
Macrophages / immunology
Mice
Mice, Inbred BALB C
Opsonin Proteins / immunology*
Phagocytosis / immunology
Polysaccharides / immunology
Quillaja Saponins
Saponins / pharmacology*
Streptococcus pneumoniae / immunology*

Substances

Adjuvants, Immunologic
Bacterial Vaccines
Immune Sera
Opsonin Proteins
Polysaccharides
Quillaja Saponins
Saponins
Quil A