Abstract
Carp acclimated to 10 degrees C gave 69k, 66k, and 62kDa light meromyosin (LMM) fragments in SDS-PAGE, while fish acclimated to 30 degrees C gave 74k, 69k, 66k, and 62kDa fragments. The microsequence analysis revealed that the 69k and 66kDa components from the 10 degrees C-acclimated carp contained an N-terminal amino acid sequence different from that of 62kDa. The four fragments from the 30 degrees C-acclimated carp showed the same sequence as that of the 69k and 66kDa components from the 10 degrees C-acclimated carp, except that the 2nd amino acid, Ala, of the 10 degrees C-acclimated LMM was replaced by Thr. DNA fragments encoding an N-terminal region of LMM were amplified by PCR or reverse transcriptase-PCR, demonstrating that the two acclimated groups further contained several amino acids substituted.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Acclimatization
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Amino Acid Sequence
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Animals
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Carps
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Chickens
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Cloning, Molecular
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Cold Temperature
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DNA, Complementary
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Electrophoresis, Polyacrylamide Gel
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Humans
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Mice
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Molecular Sequence Data
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Muscle Fibers, Fast-Twitch / metabolism
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Muscle, Skeletal / metabolism*
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Myosin Subfragments / biosynthesis*
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Myosin Subfragments / chemistry
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Myosin Subfragments / isolation & purification
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Peptide Fragments / chemistry
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Peptide Fragments / isolation & purification
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RNA, Messenger / biosynthesis
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RNA, Messenger / metabolism
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Rabbits
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Rats
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Recombinant Proteins / isolation & purification
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Sequence Homology, Amino Acid
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Temperature
Substances
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DNA, Complementary
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Myosin Subfragments
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Peptide Fragments
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RNA, Messenger
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Recombinant Proteins
Associated data
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GENBANK/D43699
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GENBANK/D43700