Molecular cloning and functional expression of a cDNA encoding a human pituitary adenylate cyclase activating polypeptide receptor

Biochem Biophys Res Commun. 1993 Nov 15;196(3):1511-21. doi: 10.1006/bbrc.1993.2423.

Abstract

A functional cDNA clone for a human pituitary adenylate cyclase activating polypeptide (PACAP) receptor was isolated from a human pituitary cDNA library. The cDNA encoded a polypeptide consisting of 525 amino acids with putative seven hydrophobic domains. Chinese hamster ovary (CHO)-K1 cells transfected with the cDNA specifically bound PACAP and mediated PACAP-triggered intracellular accumulation of cAMP, indicating that this cDNA encoded a functional human PACAP Type I receptor. This receptor was structurally related to the vasoactive intestinal peptide (VIP), secretin, calcitonin and parathyroid hormone receptors and is much more homologous to a rat PACAP receptor. Northern blot analysis revealed that PACAP receptor mRNAs were expressed mainly in the brain and widely distributed in the central nervous system.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • CHO Cells
  • Cell Membrane / metabolism
  • Cloning, Molecular
  • Cricetinae
  • Cyclic AMP / metabolism
  • DNA, Complementary / metabolism
  • Gene Expression
  • Gene Library
  • Humans
  • Kinetics
  • Molecular Sequence Data
  • Neuropeptides / metabolism
  • Neurotransmitter Agents / metabolism
  • Oligonucleotide Probes
  • Pituitary Adenylate Cyclase-Activating Polypeptide
  • Pituitary Gland / metabolism*
  • Protein Conformation
  • Rats
  • Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide
  • Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
  • Receptors, Pituitary Hormone / biosynthesis*
  • Receptors, Pituitary Hormone / genetics
  • Receptors, Pituitary Hormone / metabolism
  • Sequence Homology, Amino Acid
  • Transfection
  • Vasoactive Intestinal Peptide / pharmacology

Substances

  • ADCYAP1 protein, human
  • ADCYAP1R1 protein, human
  • Adcyap1 protein, rat
  • DNA, Complementary
  • Neuropeptides
  • Neurotransmitter Agents
  • Oligonucleotide Probes
  • Pituitary Adenylate Cyclase-Activating Polypeptide
  • Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide
  • Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
  • Receptors, Pituitary Hormone
  • Vasoactive Intestinal Peptide
  • Cyclic AMP

Associated data

  • GENBANK/D17516
  • GENBANK/L14791
  • GENBANK/L14792
  • GENBANK/L14793
  • GENBANK/L14794
  • GENBANK/L14795
  • GENBANK/L14796
  • GENBANK/L14797
  • GENBANK/L14798
  • GENBANK/U03866