Abstract
Transport of the carcinogen benzo[a]pyrene in apical membrane vesicles (AMV) from normal human intestine, was investigated. Benzo[a]pyrene transport was found in AMV throughout the small intestine, but was greatest in colon. Evidence suggesting involvement of P-Glycoprotein (P-Gp), included (1) comparable transport of P-Gp substrate doxorubicin, (2) transport stimulation by ATP and (3) transport suppression by the P-Gp inhibitor, verapamil.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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Adenosine Triphosphate / pharmacology
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Adult
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Aged
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Alkaline Phosphatase / analysis
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Aminopeptidases / analysis
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Benzo(a)pyrene / metabolism*
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Biological Transport, Active / drug effects
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CD13 Antigens
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Carrier Proteins / metabolism
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Cell Membrane / metabolism
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Colon / metabolism*
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Colon / ultrastructure
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Doxorubicin / metabolism
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Female
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Humans
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Intestine, Small / metabolism*
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Intestine, Small / ultrastructure
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Male
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Membrane Glycoproteins / metabolism
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Middle Aged
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Verapamil / pharmacology
Substances
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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Carrier Proteins
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Membrane Glycoproteins
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Benzo(a)pyrene
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Doxorubicin
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Adenosine Triphosphate
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Verapamil
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Alkaline Phosphatase
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Aminopeptidases
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CD13 Antigens