Abstract
Among the homeotic mutants with altered floral organs, two mutants of Arabidopsis thaliana, apetala3 and pistillata, and two mutants of Antirrhinum majus, deficiens and globosa, have a homeotic conversion of the floral organs in whorl 2 and 3, namely petals to sepals and stamens to carpels. We have isolated a homologue of the DEFICIENS gene from A. thaliana wild type and shown complete complementation of apetala3 mutation by introducing the isolated gene using Agrobacterium-mediated transformation. These results show that the APETALA3 is a homologue of DEFICIENS structurally and functionally. The 5'-upstream region of APETALA3 contains three SRE-like sequence, where MADS box-containing proteins are assumed to bind and regulate expression in tissue- and stage-specific manner.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing
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Amino Acid Sequence
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Arabidopsis / genetics*
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Base Sequence
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Cloning, Molecular
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Gene Expression Regulation, Developmental
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Gene Expression Regulation, Plant
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Genes, Homeobox / genetics*
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Genes, Plant / genetics*
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Genetic Complementation Test
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Genomic Library
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Molecular Sequence Data
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Mutation / physiology
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Plant Proteins / chemistry
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Plant Proteins / genetics*
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Plant Stems / chemistry
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Plants / genetics*
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Plants, Genetically Modified
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RNA, Messenger / analysis
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RNA, Plant / analysis
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Saccharomyces cerevisiae Proteins*
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Homology, Nucleic Acid*
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Trans-Activators*
Substances
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Adaptor Proteins, Signal Transducing
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HFI1 protein, S cerevisiae
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Plant Proteins
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RNA, Messenger
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RNA, Plant
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Saccharomyces cerevisiae Proteins
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Trans-Activators