Calcium binding properties of rabbit liver protein disulfide isomerase

Biochem Biophys Res Commun. 1994 Jul 15;202(1):556-61. doi: 10.1006/bbrc.1994.1964.

Abstract

We have characterized the Ca2+ binding properties of protein disulfide isomerase (PDI). It binds 19 mol Ca2+/mol protein with low affinity, which is reduced by increasing the ionic strength. Ca2+ induced conformational changes detected by UV difference spectroscopy. These Ca2+ binding properties resemble those of a sea urchin egg 58 kDa protein.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Calcium / metabolism*
  • Calcium / pharmacology
  • Calcium-Binding Proteins / chemistry
  • Calcium-Binding Proteins / isolation & purification
  • Calcium-Binding Proteins / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Isomerases / chemistry
  • Isomerases / isolation & purification
  • Isomerases / metabolism*
  • Kinetics
  • Liver / enzymology*
  • Molecular Weight
  • Protein Binding
  • Protein Conformation / drug effects
  • Protein Disulfide-Isomerases
  • Rabbits
  • Spectrophotometry, Ultraviolet

Substances

  • Calcium-Binding Proteins
  • Isomerases
  • Protein Disulfide-Isomerases
  • Calcium