Abstract
We have characterized the Ca2+ binding properties of protein disulfide isomerase (PDI). It binds 19 mol Ca2+/mol protein with low affinity, which is reduced by increasing the ionic strength. Ca2+ induced conformational changes detected by UV difference spectroscopy. These Ca2+ binding properties resemble those of a sea urchin egg 58 kDa protein.
MeSH terms
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Animals
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Calcium / metabolism*
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Calcium / pharmacology
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Calcium-Binding Proteins / chemistry
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Calcium-Binding Proteins / isolation & purification
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Calcium-Binding Proteins / metabolism*
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Electrophoresis, Polyacrylamide Gel
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Isomerases / chemistry
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Isomerases / isolation & purification
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Isomerases / metabolism*
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Kinetics
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Liver / enzymology*
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Molecular Weight
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Protein Binding
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Protein Conformation / drug effects
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Protein Disulfide-Isomerases
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Rabbits
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Spectrophotometry, Ultraviolet
Substances
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Calcium-Binding Proteins
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Isomerases
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Protein Disulfide-Isomerases
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Calcium