Pituitary adenylate cyclase-activating polypeptide (PACAP) stimulates melatonin synthesis from rat pineal gland

Brain Res. 1993 Feb 12;603(1):148-52. doi: 10.1016/0006-8993(93)91313-h.

Abstract

The effect of pituitary adenylate cyclase polypeptide (PACAP) on rat pineal was examined. PACAP stimulated melatonin release from cultured dissociated pinealocytes with a 10(4) higher potency than isoproterenol (EC50 were 30 pM and 250 nM, respectively). The 10(-9) M PACAP stimulation was not inhibited by 5 x 10(-6) M VIP antagonist whereas that of 10(-9) M VIP was reduced by 54%. Kinetic analysis of melatonin release indicated that PACAP acts postsynaptically via receptor activation.

MeSH terms

  • Animals
  • Cells, Cultured
  • In Vitro Techniques
  • Isoproterenol / pharmacology
  • Male
  • Melatonin / biosynthesis*
  • Neuropeptides / pharmacology*
  • Neurotransmitter Agents / pharmacology*
  • Perfusion
  • Pineal Gland / drug effects
  • Pineal Gland / metabolism*
  • Pituitary Adenylate Cyclase-Activating Polypeptide
  • Rats
  • Rats, Wistar
  • Receptors, Gastrointestinal Hormone / antagonists & inhibitors
  • Receptors, Vasoactive Intestinal Peptide
  • Stimulation, Chemical
  • Vasoactive Intestinal Peptide / antagonists & inhibitors
  • Vasoactive Intestinal Peptide / pharmacology

Substances

  • Adcyap1 protein, rat
  • Neuropeptides
  • Neurotransmitter Agents
  • Pituitary Adenylate Cyclase-Activating Polypeptide
  • Receptors, Gastrointestinal Hormone
  • Receptors, Vasoactive Intestinal Peptide
  • Vasoactive Intestinal Peptide
  • Melatonin
  • Isoproterenol