Categorization of clinical isolates of Helicobacter pylori on the basis of restriction digest analyses of polymerase chain reaction-amplified ureC genes

J Clin Microbiol. 1993 May;31(5):1334-5. doi: 10.1128/jcm.31.5.1334-1335.1993.

Abstract

Restriction endonuclease analyses of a 1.1-kb polymerase chain reaction-amplified portion of the ureC gene from Helicobacter pylori were used to group or to differentiate 21 clinical isolates. Isolates were placed into 4 groups after HindIII digestion alone, and placement was expanded into 15 groups after isolates were digested with AluI and PvuI.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques*
  • Base Sequence
  • DNA, Bacterial / genetics
  • Genes, Bacterial*
  • Helicobacter Infections / microbiology
  • Helicobacter pylori / classification*
  • Helicobacter pylori / genetics*
  • Helicobacter pylori / isolation & purification
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Urease / genetics*

Substances

  • DNA, Bacterial
  • Urease