Comparison of methods for specific depletion of ATP in Salmonella typhimurium

Appl Environ Microbiol. 1993 Oct;59(10):3509-12. doi: 10.1128/aem.59.10.3509-3512.1993.

Abstract

Three methods of ATP depletion in Salmonella typhimurium were compared. ATP concentrations were lowest after arsenate treatment. Arsenate or alpha-methylglucoside-plus-azide treatment nonspecifically lowered all nucleotide triphosphate levels. Histidine starvation in a hisF mutant was relatively specific for ATP depletion and therefore has potential in distinguishing ATP-dependent processes from processes dependent on other nucleotides.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphate / metabolism*
  • Arsenates / pharmacology
  • Azides / pharmacology
  • Bacteriological Techniques
  • Evaluation Studies as Topic
  • Genes, Bacterial
  • Histidine / genetics
  • Histidine / metabolism
  • Kinetics
  • Methylglucosides / pharmacology
  • Mutation
  • Nucleotides / metabolism
  • Salmonella typhimurium / drug effects
  • Salmonella typhimurium / genetics
  • Salmonella typhimurium / metabolism*
  • Sodium Azide

Substances

  • Arsenates
  • Azides
  • Methylglucosides
  • Nucleotides
  • Histidine
  • methylglucoside
  • Adenosine Triphosphate
  • Sodium Azide
  • arsenic acid