Abstract
Three methods of ATP depletion in Salmonella typhimurium were compared. ATP concentrations were lowest after arsenate treatment. Arsenate or alpha-methylglucoside-plus-azide treatment nonspecifically lowered all nucleotide triphosphate levels. Histidine starvation in a hisF mutant was relatively specific for ATP depletion and therefore has potential in distinguishing ATP-dependent processes from processes dependent on other nucleotides.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphate / metabolism*
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Arsenates / pharmacology
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Azides / pharmacology
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Bacteriological Techniques
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Evaluation Studies as Topic
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Genes, Bacterial
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Histidine / genetics
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Histidine / metabolism
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Kinetics
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Methylglucosides / pharmacology
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Mutation
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Nucleotides / metabolism
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Salmonella typhimurium / drug effects
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Salmonella typhimurium / genetics
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Salmonella typhimurium / metabolism*
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Sodium Azide
Substances
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Arsenates
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Azides
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Methylglucosides
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Nucleotides
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Histidine
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methylglucoside
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Adenosine Triphosphate
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Sodium Azide
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arsenic acid