Defining the common subtypes of HLA A9, A10, A28 and A19 by use of ARMS/PCR

Tissue Antigens. 1993 Aug;42(2):91-9. doi: 10.1111/j.1399-0039.1993.tb02243.x.

Abstract

We describe sequence-specific primer (SSP) combinations for use in a one-step polymerase chain reaction (PCR) typing system to determine HLA-A locus subtypes of A9 (A23, A24), A10 (A25, A26, A43), A28 (A*6801, A*6802, A*6901) and A19 (A*2901, A*2902, A*3001, A*3002, A31, A32, A33) from genomic DNA. SSP's were designed on the basis of the amplification refractory mutation system (ARMS) in which a mismatch at the 3' residue inhibits non-specific amplification. The SSP combinations described extend our low-resolution typing system, to provide a high-definition typing of the HLA-A locus.

MeSH terms

  • Alleles
  • Base Sequence
  • DNA Primers*
  • Genes, MHC Class I*
  • HLA-A Antigens / genetics*
  • Histocompatibility Testing / methods*
  • Humans
  • Molecular Sequence Data
  • Mutation
  • Pedigree
  • Polymerase Chain Reaction*
  • White People / genetics

Substances

  • DNA Primers
  • HLA-A Antigens