Abstract
Protein kinase C(PKC) and protein kinase A(PKA) purified from rat liver were incubated with (-)- and (+)-gossypol. The activity of PKC was significantly inhibited in a concentration-dependent manner by both (-)-gossypol and (+)-gossypol. (-)-gossypol was found to inhibit type I PKA, whereas the inhibitory action of (+)-gossypol for type I PKA was less potent. Both (-)-gossypol and (+)-gossypol showed inhibition for type II PKA only at high concentration. These results suggest that administration of gossypol might impair cellular signal transduction.
Publication types
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Comparative Study
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cyclic AMP-Dependent Protein Kinase Type II
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Cyclic AMP-Dependent Protein Kinases / isolation & purification
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Cyclic AMP-Dependent Protein Kinases / metabolism*
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Dose-Response Relationship, Drug
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Gossypol / pharmacology*
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Liver / chemistry
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Male
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Protein Kinase C / isolation & purification
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Protein Kinase C / metabolism*
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Rats
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Rats, Wistar
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Stereoisomerism
Substances
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Cyclic AMP-Dependent Protein Kinase Type II
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Cyclic AMP-Dependent Protein Kinases
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Protein Kinase C
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Gossypol